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DeCS
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Descriptor Inglés:
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Chromatin Immunoprecipitation
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Descriptor Español:
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Inmunoprecipitación de Cromatina
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Descriptor Portugués:
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Imunoprecipitação da Cromatina
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Sinónimos Inglés:
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Immunoprecipitation, Chromatin
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Categoría:
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E05.393.170
E05.478.605.160
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Definición Inglés:
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A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. |
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Nota Histórica Inglés:
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2005
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Calificadores Permitidos Inglés:
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Número del Registro:
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38826
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Identificador Único:
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D047369
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Ocurrencia en la BVS:
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Similar:
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DeCS
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